Overview

The Carroll lab has an established record of attacking fundamental problems in redox biology through a powerful, interdisciplinary approach that integrates synthetic chemistry with proteomics, biochemistry, and cell biology. An overarching goal of our research program is to understand the biological chemistry and molecular mechanisms of redox-based cellular regulation and signal transduction, with particular emphasis on the role of cysteine oxidation, a ubiquitous and conserved mechanism for controlling protein function. We are also exploring the therapeutic potential of redox-regulated protein function by developing an entirely new class of inhibitors that targets oxidized cysteine residues of key proteins involved in human disease. Ultimately, our goal is to accelerate the discovery of key regulatory nodes of redox-signaling networks, profile changes in protein cysteine oxidation associated with disease, and harness this information for the development of new diagnostic and therapeutic approaches.

Chemical Tools
Redox Signaling
Thio Proteomics
Covalent Inhibitors
Biomarker Discovery
Antioxidant Therapy
Protein Engineering
Enzyme Mechanism
Antibiotic Resistance

Training

Students in the lab receive broad-based training in experimental techniques ranging from synthetic chemistry and mass spectrometry to cellular and in vivo animal studies. Representative skill sets and expertise in the group are given below, and students are encouraged to take multiple apporaches to ask and answer new scientific questions.

  • Chemical tool development: Synthetic chemistry with analytical characterization

  • Cell culture: Mammalian cell lines, bacteria, and primary cultures

  • Proteomics: Solid-phase capture, fractionation, LC-MS/MS, bioinformatics

  • Molecular imaging: Confocal microscopy and flow cytometry

  • Gene discovery: Activity-based protein profiling

  • Animal studies: Mouse physiology

  • Molecular biology: Cloning, transfections, RNAi, PCR, and CRISPR

  • In vitro biochemistry: Protein preparation, purification, and protein engineering

Copyright 2016 Kate Carroll Lab

The Scripps Research Institute